Tabriz University of Medical Sciences Advanced Pharmaceutical Bulletin 2228-5881 3 2 2013 12 30 Thin Layer Chromatographic Analysis of Beta-Lactam Antibiotics 367 371 10.5681/apb.2013.059 EN Gabriel Hancu Brigitta Simon Hajnal Kelemen Aura Rusu Eleonora Mircia Arpad Gyeresi Journal Article 10.5681/apb.2013.059 2013 02 07 Purpose: The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. Methods: Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the separation of the studied beta-lactamins. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the optimization of the analytical conditions. Results: No system will separate all the beta-lactams, but they could be identified when supplementary information is used from color reactions and/or by using additional chromatographic systems. Conclusions: The right combination of solvent system and detection method allows the identification of the studied penicillins and cephalosporins and can be successfully used in the preliminary analysis beta-lactam antibiotics.