Abstract
Purpose: The use of drug delivery on nanoparticles has been expanded to improve the efficacy of treatment. The aim of the present study was to investigate the effect of 7‑hydroxy‑4‑methylcoumarin (hymercromone) loaded on synthesized layered double hydroxide (LDH)/polycaprolactone (PCL)/gelatin (Gel) nanofibrous scaffolds on the death of the B16F10 cancer cell line. Methods: The LDH/hymecromone nanohybrid was prepared using a co-precipitation technique, then incorporated into a PCL/GEL polymer solution and fabricated via electrospuning. The scaffold was characterized in terms of its phase, morphology, and elemental composition using X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy, respectively. Cell viability was evaluated through the MTT assay, while a Calcein AM/PI dual-staining kit was employed for further analysis using confocal microscopy. Results: The findings showed that the LDH/hemichromone nanohybrid was properly synthesized and incorporated into the nanofibrous scaffold. It was also found that the addition of LDH nanoparticles to the PCL/Gel scaffold improved its mechanical strength and elongation at break. MTT results revealed that the survival rate of B16F10 cells treated with drug loaded on PCL/Gel/LDH scaffolds for 72 hours was less than 24 hours. Confocal microscopy results also confirmed the MTT results and showed that more cell death occurred at 72 hours than at 24 hours. As the depth of the scaffold approached, the number of cells gradually decreased. Conclusion: It appears that PCL/Gel/LDH scaffolds can be suitable candidate for drug loading for therapeutic application in cancer cells.